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 On flowcell, amplification-free RNA-seq |
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Jan 31, 2010 - 8:27 PM - by krobison
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(I like my title better :-)
Nat Methods. 2010 Feb;7(2):130-2. Epub 2010 Jan 17.
FRT-seq: amplification-free, strand-specific transcriptome sequencing.
Mamanova L, Andrews RM, James KD, Sheridan EM, Ellis PD, Langford CF, Ost TW, Collins JE, Turner DJ.
[1] The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK. [2] These authors contributed equally to this work.
We report an alternative approach to transcriptome... [Read More]
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2 Replies | 349 Views
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Illumina's new sequencers: HiSeq, GAIIx, GAIIe or iScan-Seq |
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Jan 20, 2010 - 4:10 AM - by james hadfield
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So Illumina now have four sequencers available; GAIIx, HiSeq, GAIIe and iScan sequencing module (are they still doing this?). All offer exactly the same applications, biochemistry and data. But they differ in output.
The GAIIx, GAIIe and iScan seq module (? but I will not really consider it here any more) use the same flowcells but generate very different amounts of data. How do they do this? Is it a smaller camera or fewer tiles? Could it be lower cluster density? Or is the data slimmed down computationally? With any of these I suspect there may be possibilities to increase... [Read More]
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16 Replies | 1,466 Views
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Illumina's new sequencer: HiSeq 2000 |
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Jan 12, 2010 - 7:27 PM - by mccullou
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Wow GAII just came out last year, they just now got the reagents QC under control after their SNAFU and now this. . .
http://www.illumina.com/systems/hiseq_2000.ilmn
How different can this be? It uses the cBot (which I have but there are no reagents to buy yet) so I know the cluster station is more walk away, but is the sequencing chemistry and engineering so much better as to:
"obtain ~30x coverage of two human genomes in a single run for under $10,000 (USD)* per... [Read More]
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14 Replies | 1,629 Views
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