- Nebulization, other shearing methods
- ChIP-Seq problems with library generation
- Covaris/Hydroshear
- ChIP seq sample prep for Illumina
- New DNA shearing technology?
- fomalin fixed paraffin embedded sample prep?
- question on DNA fragment size after shear the DNA (in Chip-seq)
- Enrichement and Picogreen concentrations?
- Caution about nebulization for sample prep
- Discrepancies between PicoGreen and qPCR in GA libraries
- Bias toward G in first nucleotide in sequence?
- WGA pre-Next Gen Sequencing
- High-Throughput DNA Shearing
- hybridization platform
- [Sample prep] Enrichment and aspecific control use with qPCR
- Generating polyclonal antibodies from peptides for ChIP-Seq
- CHiP-SEQ size and SOLiD
- need help with klenow
- solexa adapter ligation question
- No peaks in my ChIP Seq samples
- Sanger publishes amplification-free Illumina library prep paper
- Phi29 polymerase problem while WGA
- Amplicon sequencing: Method to prevent end over-representation & improve uniformity
- Bioanalyser traces + QC postPCR. ChIPseq Illumina protocol
- How do you prevent contamination?
- Custom Illumina adapter synthesis
- gel extraction protocols
- Library quality check using known genes
- Using Covaris S2 for RNA shearing
- sequencing 12Mb region
- Adaptor technologies comparison
- RNA-Seq: cDNA prep and fragmentation questions
- 3'UTR library or random primed cDNA library for quantification?
- Question of small quantity of cell or single cell sequencing
- HELP with preparing DNA for plant ChIP seq
- fragmenting amplicons?
- Standardized vs normalized libraries
- Sequencing Illumina mate pair libraries beyond 36bp
- Roche/454 Titanium Amplicon sequencing?
- Anyone use low concentration for ChIP-seq prep?
- ChIP DNA Quantitation with Low-Range PicoGreen Standard Curve
- Sequencing random transgene insertions
- HydroShear - 8 kb PE library
- Streptavidin bead buffers for mate pair prep kit
- Question about 350bp band showing up after PCR
- Homopolymer tract deletions in Illumina Library Preparation
- Ligase for DIY GAII library preps
- RNA-Seq when not using the Illumina Kit
- Shoulders/humps seen in DNA library
- Concentration of DNA to be denatured, loaded onto flowcell
- What is at the ends of sonicated DNA?
- Automating library prep for 454/GAII
- Target enrichment from pooled libraries
- Need Advise on the mRNA sequencing
- Is possible to stain with SYBR green
- illumina primer/adaptor concentrations
- New fragmentation methods
- Where does all the DNA go?
- Using of Rnase A à 65°C- Chip_Seq
- strand specificity
- Immobilization beads and poor libraries--anyone see a 100bp band leak through?
- Better library construction protocols for better coverage/assembly
- Covaris S2: Chromatin shearing for ChIP(-seq?)
- What fragment size do you use for your Illumina libraries?
- Illumina small RNA ladder and NEB miRNA marker
- Agilent Bioanalyzer refuses to size bands
- 454: Watch out when multiplexing MID 16 and 18!
- chip-seq cluster,s number
- help: time of adapter ligation
- the minimal amount of DNA required for deep-sequencing platforms!
- Agilent SureSelect enrichment
- Roche 454 for bacterial ChIP
- Illumina mRNA-seq protocol
- Quality testing of ChIP-seq library prior to sequencing.
- Sera-mag buffers from Illumina RNA-seq kit
- Number of PCR How many PCR cycles to enrich adapter-modified DNA fragments
- Removal of large fragments?
- titrate adapter & extra bands in library
- chip-seq
- Biomek fx 'how to'
- illumina library prep troubleshooting guide?
- genomic prep question
- need help with library prep
- Double AMPure size selection in Titanium 3kb span paired end library
- Ampure versus Ampure XP
- question on making libraries from pcr products
- ampure purification of illumina libraries
- Targeted Sequencing - How are you doing sample prep for your targeted sequencing prj?
- Covaris parameters to obtain ~200bp fragments?
- library prep by transposition
- Bioanalyzer on pre-prepped ChIP-seq samples
- library preparation cost
- Total RNA quality (Bioanalyzer trace attached)
- Covaris parameters to obtain 300~600bp fragments?
- RNA seq libraries from few hundred cells
- illumina alternative v1.5 protocol for small rna seq vs. the standard protocol
- Extreme nucleotide bias at fragment ends of Illumina mate pair library
- Is that possible to get library ~200 from Chip DNA ~500
- Heat shock ssDNA generation-Illumina
- Covaris RRL approach
- PE mRNA seq library construction
- Homemade Illumina mRNA-SEQ protocols
- Shearing Chromatin using Covaris
- mRNA fragmentation
- Bioanalyser results???
- Primer extension capture
- 4% agarose gels?
- NEBNext GAII Paired End Prep
- Spiking phiX into multiplexed libraries as well as barcode sequences
- Chromatin shearing for ChIP-Seq using Covaris S2
- RNA sample quality for 454 transcriptome sequencing
- Rationale for Superscript II RT use in Illumina 1st strand synthesis
- bioanalyzer image
- FLX vs Titanium library
- In solution capture of whole chunks of genomic DNA?
- insert size for illumina 72-SE
- Looking for covaris in San Diego-La Jolla
- Mate-Pair biotinylation
- Mate pair, high GC
- Low throughput DNA quantitation?
- Insert size with Agilent SureSelect
- alternatives to sera-mag bead purification
- insert size
- which qPCR to use for quantitating lib?
- Linker Bias in 454 Paired-End Libraries
- Isolation of genomic DNA
- Ampure XP inconsistency
- RNA-seq to detect expressed SNPs
- actual paired-end distances
- Can Oragene-DNA be used for targeted capture and clonal sequencing?
- Total RNA quality for 454
- NGS App-Enabling Up-Front Molecular Biology
- Ratio in Adapter-Ligation
- Sonicator
- Shearing methods for 454 8kb libraries
- YACs as a solution to repeat regions
- Strange higher size amplicon in ChIP seq Library!!
- RNA-seq library prep problem
- Restriction digest of RNA /DNA hybrid
- Covaris 96 well format
- Target Enrichmnet In-Solution
- RNA fragmentation
- QC of cDNA library prep for GA
- Strange higher band in Bioanalyzer after size selection
- Fragmentation of RNA or cDNA
- New to ChIP-seq
- GAIIx versus HiSeq Libraries
- Problems with Modified Paired End Sample Prep Protocol
- Ampure XP automation
- Successfull DNA conversion by EZ DNA Methylation-Gold kit???
- Inexpensive paired-end protocol
- In-solution SureSelect capture library to prepped sample ratio
- bad library prep results
- PCR Clean up - 96 well
- problems with 454 library prep
- Chip-seq sample prep for bacteria
- Sample quality for RNA_SEQ
- Pooling the samples
- Adaptors for ChIP-seq libraries
- RiboMinus yields
- SPRI-TE Illumina library preps
- Illumina Library Prep Issue
- Low concentration GA library
- 454 Rapid kit in metgenomics
- Baits length for exome capture? (Nimblegen and Aglient)
- normalizing small RNA libraries
- Mate-pair library prep for Illumina
- Automated size fractionation of samples or libraries
- Library quantification: opinions?
- Better to risk low concentration library or further amplification?
- mrna seq or directional mrna seq
- mag beads?
- Library prep using chromosome sorted DNA
- PW1 in SBS Sequencing Kit v2 consist of what components?
- Does anyone have experience of fixing mouse testis
- Call for European SME in target enrichment
- Ampure versus Qiaquick for small amounts of DNA
- cDNA fragmentation for SOLiD sequencing
- low 260/230 ratio for RNA-seq sample
- Generation of 3' directed cDNA libraries
- Insert size for paired end sequencing for identification of structural variation
- conventional sequencing
- nextera
- Agilent SureSelect Human All Exon 50MB kit vs 38MB kit
- sequalprep normalization of amplicons for titanium
- Paired end Illumina prep problems?
- Does TE buffer impact on covaris shearing?
- QuBit fluorimeter vs. Epoch?
- SMART Concatemeres
- Pippin Prep for size selection = win
- conversion of total DNA (ng) into nM
- ChIP library prep results (Illumina)
- qubit/picogreen
- Size selction
- LCL vs whole blood samples? (shearing differences)
- Help with denaturing low concentration Illumina PE Library
- Target enrichment and CNV analysis
- three-read barcoding and adapters
- Multiplexing prep.
- What's your favourite qPCR kit for Illumina library QC?
- Repeat Sequence Library Generation
- Barcode before exome capture
- Whole genome amplification prior to ChIP-Seq
- sequence capture and 454
- double AMPure size selection
- indexing for Hiseq
- mRNA library problems
- Chip-seq: Comment on shearing
- Problems with Nextera
- Do you size select??
- ScriptSeqTM mRNA-Seq Library Preparation Kit
- RNAseq from single cell RT?
- RNase treatment
- Do we need controls for NGS?
- Library prep from single-stranded DNA?
- need some help..covaris and dilutions
- high throughput DNA size selection?
- 4C libraries
- Sera-Mag oligo-dT beads
- Experience with nugen ovation 3 dge System
- tRNA sequencing?
- Bait tiling
- how much DNA is required for library preparation?
- Need help: Weird size selection
- Covaris fragmentation with 100bp reads
- Multiplexing without indexing sequencing
- Misonix Sonicator 4000
- Biomek FX Automation
- TruSeq adapter sequences
- GEX Second Strand Buffer
- Illumina Human Exome vs Agilent Human Exome
- Ampure XP Hack
- Calibration beads
- Capillary DNA sequencing
- DSN normalization
- DNA contamination in mRNA seq prepare
- Have someone use the Misonix 4000 for solexa
- Multiplex adapters and indexes for Illumina HiSeq
- bacterial RNA sequencing and rRNA removal
- ChIPseq: Strange Peaks after Gel Extraction
- Agencourt Ampure purification kit - exp date
- covaris shearing settings for paired end
- Yeast ChIP-seq; Sonication/Bioanalyzer question
- Size selection of ChIP-Seq library
- massive multiplex PCR-library for 454-sequencing
- RNA-seq library prep problems
- how to prepare home-made adapters ?
- EZ bead libraries/beads deposition in 5500
- Agilent Bioanalyzer 2100 detects no DNA
- Edit distance sequence tags available (link within)