- Nimblegen arrays for targeted genome enrichment
- Getting Newbler
- 454 expected sequences and Tag for amplicon sequencing
- Nimblegen officially describes "SeqCap" genome decomplexing service
- New journal articles
- Help with 454 bar coded samples
- chromosome rearrangements
- High Short Reads %
- new 454 system
- 454 amplicon sequencing - gene family with large number of paralogs
- Starting the analysis of 454 data
- GS-FLX reagent shelf lives
- 454 Configuration Management
- Capture and 454
- Tech Summary: Roche's 454 GS20 / FLX / Titanium
- SNP calling on 454 data
- Mixed fragment size normalized cDNA sequencing
- Plasmid contamination in Long Tag Paired End library
- 454 Assembly viewer
- Deletion detection on seq-cap experiments
- Outlier in Newbler
- 454 homopolymer errors or????
- DNA Shearing for 454
- Titanium software (MPI mode)
- 454 Maintenace wash
- 454: Unmapped contigs after Reference Assembly
- Nimblegen Seq Capture Vs RainDance Technologies
- Depth viewer for 454 data.
- idle 454 ?
- gsAssembler / newbler hangs during (large?) assembly
- 454 Library / Emulsion / Instrument Protocols (October 2008)
- How to align contigs?
- question about coverage, .ace file.
- Roche's gsMapper
- How to explain this scenario ?
- 454 assemly
- launching gsAssembler in Fedora
- Homopolymer error in 454 sequencing data
- 454 sequencing data coverage
- newbler background
- sfffile converting quality values
- System requirements linux comp. for off-machine assembly/analysis
- compare GSMapper and BLAT
- Uniform droplet size?
- Recommendation from 454 for Titanium PE/Shotgun
- gsRunProcessor: error with v.2.0.00.22
- Roche Software - Slow GUI
- Is 10x coverage suitable for resequecing 1Gb genome using 454?
- Comparing SNP/indel detection tools on 454
- Tween Concentration in Maintenance Wash
- Poor enrichment...defective enrichment beads?
- Want to generate more data to fill in gaps
- Short reads in Titanium
- Alternative sequence enrichment methods
- Identifying collapsed repeats and other misassemblies
- Titanium kit - short reads
- Seqcap service
- GS FLX & Illumina
- 454 for RNA-Seq for 2 group comparison
- Duplicate reads ("same start" reads) in 454 FLX/Titanium shotgun runs
- cDNA synthesis kits with titanium
- 454 titanium sequence company
- 454 homopolymer error rate
- what's clipping point?
- getting reads not filtered?
- Poly(A) Tail treatment?
- postAnalysisScript.sh
- @454Sequencing on twitter!
- Roche soft Error: "At least one read sequence...."
- Turn off KeyPass Filter?
- Amplicon sequencing using Titanium technology
- Titanium problems
- Newbler --- classify many reads as Repeat
- Question --Incremental assembly using Nembler
- emPCR robot?
- Assessing a 454 run?
- 454 Vs Sanger
- gsMapper issues
- Control read variation
- GS Mapper output to consed shows dimmed ends with good sequence?
- Lims
- Titanium Runs
- small DNA sample library prep
- Summary Statistics in Newbler?
- advice for de novo assembly of plant genome using 454
- Titanium PE yields?
- emulsion droplets
- UTP and emPCR
- Titanium: Does it suck?
- Titanium upper and lower case bases
- GS FLX data analysis software manual
- Size Selection Tricks??
- Anyone know what the fill-in enzyme is?
- emPCR additive
- Roche software update 2.0.01.12
- Buying reagents from outside source to replace the 454 DNA library kit
- Different NextGen platforms sharing the same space
- primers and adaptors
- gsMapper to SAM/BAM
- gsassembler.
- amplicon size for titanium?
- gsMapper and compiling unmapped reads
- Number of Reads in 454 Library
- Finding Raw 454 Data
- Low Molecular Weight sample and primer dimers
- Replacement/generic Mock Amplification Mix
- suppression subtractive hybridization and 454
- Error Bar in 454's Flowgram
- Sequencing repeats: how to?
- 454 assembling against reference genome
- 454 runAssembly contig lengths
- miRNA in transcriptome or genome datasets?
- Software hangs on maintenance wash
- Renaming reads within SFF files
- 1/2 read and coverage?
- seqence on DNA capture bead of Titanium diff from FLX Standard?
- How quantitative is contig coverage?
- pyrotag process
- ReferenceMapper: Annotation files & known SNPs
- Difference between 454 pair end library and 454 standard library
- How to determine 454 paired end adaptor sequence
- what's the retro-fitted reaction in pyrosequencing
- 454 titanium emulsion PCR problems?
- OTF Ti signal processing
- Discussion and explanation about Roche's 454 GS20 / FLX / Titanium?!
- improper bead deposition
- Titanium - high fraction of short reads
- non paired adapters in 454 data
- MIRA:454 scaffolds polishing by Illumina reads discussion
- How to get started analysing 454 data
- gsAssembler v2.0.1 red X's in parameters
- REM e System for enrichment
- PCR Primer Design Software for Gap-closure
- Ti vs. FLX library construction
- Running assisted assemblies with runAssembly
- How are raw well numbers determined?
- emPCR additive
- Structural Variation with 454 Reads
- various SMART cDNA methods for transcriptome sequencing
- Enrichment with AGILENT's Sureselect target enrichment system
- Roche GS Junior mini 454 coming 2010
- WGS in one region, Amplicons in another
- Real biological 454 data publicly available for benchmarking
- emulsion pcr - one template-fragment per bead?
- 3-5% more seq from new Roche software
- read length of GS FLX Titanium
- New 454 emulsion creation
- Roche 454 Paired End with Covaris Shearing
- sff 454 files into fasta
- Raw wells?
- Amplicon library titration
- N25 & N75 sequence index
- Does splitting run reduce read number?
- Control DNA fragments sequences on titanium
- Using multiple MIDs in titanium sequence runs
- newbler cpu core usage
- PyroNoise on Mac OS X?
- Performing Hybrid Assemblies With Newbler
- trimming issue
- Newbler assembly - excluding specific reads?
- gsMapper contigs
- Rubber gasket vs. Molecular Markers
- Rapid Libraries
- Paired-end & shotgun reads
- mira3 output
- Removing primers
- mira parameters for SNP search in transcriptome de novo
- real world 454 sequencing data analysis examples?!
- Extracting contig info and singletons
- Amplicon and other library types in the same run?
- Homopolymer run errors, polyA bias
- Paper: Fully automated process for barcoded libraries for 454
- Target Sequence Enrichment 454
- Error using sff_extract.
- Flowgram signals to quality score
- coverage for amplicon seq?
- 454 Consumable Deliveries
- 454 quality score, z-score,..
- rearreangment points
- GS FLX Titanium series and small fragment
- 454assembly using Newbler: job time expectation?
- sequencing gene more than 10kb
- New England Biolabs Reagents
- coverage
- Newbler 2.3 on Ubuntu- Anyone?
- rapid cDNA library normalization?
- Roche Gs* software
- Question about evaluation of 454 sequence
- Calculation of Homopolymer errors
- standard operating procedure 454 analysis
- Detection of alternative splicing events from 454 output
- primer seq for assembly?
- Titanium Amplicon run.
- RAPID Libraries adaptor+key sequences
- best primers for 16S amplicon sequencing
- 454 Detection Probability
- Sequencing of E. coli strains
- Rapid Library Kit contents
- 454 Roche Titanium Run Costs
- Targeted sequencing
- Filtering clonal reads
- 454 supply issues?
- Newbler's algorithm -> overlap or de Brujin graph?
- standard chemistry
- 454 and cluster operating systems - patching Fedora
- problems about 454 transcription data analysis
- 16 lane run leakage
- What to do with leading T's and G's?
- Contigs from gsAssembler / newbler
- Singletons -Amplicon sequencing
- Using sff tools to Extract Read Subsets
- Problems cDNA sequencng
- Bead preparation for "GS Junior" with ABI EZ Bead Dystem
- sffinfo -s inputfile
- something about my 454 result
- Genome Sequencer Data Analysis software & CygWin
- Alternatives to sfffile & sffinfo
- Nested PCR amplicons
- Fluidigm Access Array
- CPB correction with emPCR format change
- Overcalled A's in my 454 data
- PyroNoise -DAT files
- FLX vs Titanium, Lib-L vs Lib-A, Amplicon Libraries = confusion
- Pool amplicons on a plate
- low cDNA concentration after the RAPID protocol
- running amplicons together with non amplicon lanes
- runAssembly error
- Memory Usage in Newbler 2.3
- fasta+qual = sff??
- LMW band in paired end libraries?
- SFF Read names
- problem with the ligation of adaptator- Rapid library
- Completely new to this and out of my depth
- Quantitative pyrosequencing?
- Rapid library prep and Nimblegen enrichment
- "rapid" vs "general" MID adapters
- library: control signal ratio
- gsMapper problem
- GS Junior - bead issues.
- Estimating genome size and coverage
- 454 assembly of repetitive region
- Access Array (platform from Fluidigm) for 454 library preparation
- Going from biotinylated primer to unlabeled primer in emPCR
- 454 software installation
- Quantification and between run replication
- calculating expected reads
- SureSelect and Rapid MID Libraries
- 454 Titanium error rate ?
- Delivery problem for emPCR kits
- HPLC purification of 454 fusion primers