Hi everyone,
I'm interested in your opinion on performing a ChIP-Seq experiment using 100bp paired-end reads on a HiSeq.
If sequencing costs is not so much an issue, would you use this setup? What would be the ideal fragment size for the library? What do you think of loosing resolution due to the longer reads?
Any helpful comment is much appreciated since I'm a complete beginner in this field.
Thanks a lot!
I'm interested in your opinion on performing a ChIP-Seq experiment using 100bp paired-end reads on a HiSeq.
If sequencing costs is not so much an issue, would you use this setup? What would be the ideal fragment size for the library? What do you think of loosing resolution due to the longer reads?
Any helpful comment is much appreciated since I'm a complete beginner in this field.
Thanks a lot!
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