Hello,
I am currently setting up my first SOLiD sequencing experiment, and wondered if anyone had any information regarding the recommended number of reads per sequence.
I am looking at ~20bp bovine microRNA and am likely to find between 250-400 unique sequences out of the RNA that I am sequencing, with a total of 10 separate samples to be sequenced at once. My options are one quarter of a plate, which I am told is equivalent to about 25 million reads, or a half of a plate for 50 million reads.
I can't seem to find any papers that outline this process, so was wondering if anyone knew how many reads per sequence is considered necessary to have good quality data?
Thanks!
I am currently setting up my first SOLiD sequencing experiment, and wondered if anyone had any information regarding the recommended number of reads per sequence.
I am looking at ~20bp bovine microRNA and am likely to find between 250-400 unique sequences out of the RNA that I am sequencing, with a total of 10 separate samples to be sequenced at once. My options are one quarter of a plate, which I am told is equivalent to about 25 million reads, or a half of a plate for 50 million reads.
I can't seem to find any papers that outline this process, so was wondering if anyone knew how many reads per sequence is considered necessary to have good quality data?
Thanks!
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