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  • Susanna5
    Junior Member
    • Apr 2013
    • 5

    #1

    Downloading multiple SRA runs

    Hello, I'm having some difficulties downloading multiple runs from an experiment in SRA. I don't want *all* the runs, or all the data from the experiment. I just want a selection I can give myself. I've googled about, and tried the idea of saving the selected runs to a cvs file by file>summary. and then running this command:
    sed 1d sra_result.csv | cut -f14 | wget -i-

    after observing the error of no URL, I checked the csv file summary, thinking I got the wrong column. Opening the csv file showed the runs, and the 14th entry was indeed the URL, but the entire file was not separating properly. I then added the separator to the beginning of the file:
    sep=","
    That still didn't work. It seems a novice problem, has anyone had it and/or know how to solve it?

    I know of aspera, but that also seemed to be single files from the download guide example:
    [email protected]:2...96072.lite.sra

    (I do have aspera installed) What I've tried is downloading a directory, so something like:
    [email protected]:22/sra/sra-instant/reads/ByRun/litesra/SRR/SRR096
    and also (maybe silly but getting desperate) tried this
    [email protected]:22/sra/sra-instant/reads/ByRun/litesra/SRR/SRR096/SRR096072-SRR096075
    trying to use their download example for my own purposes..
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Try this:

    Code:
    wget -r -nd -nH ftp://ftp-trace.ncbi.nlm.nih.gov/sra/sra-instant/reads//ByRun/litesra/SRR/SRR096
    This will get all accessions # in the SRR096 directory.

    Comment

    • muthu545
      Member
      • Jul 2011
      • 32

      #3
      Hi,

      The Code suggested by GenoMax is very useful. I'm just providing a modification of the code:

      Store the SRR096001-SRR096999 #s that would want to download in a file:
      for example:
      cat > SRR_2_download
      SRR096023
      SRR096072
      SRR096074

      for i in $(cat SRR_2_download);do wget -r -nd -nH ftp://ftp-trace.ncbi.nlm.nih.gov/sra/sra-instant/reads/ByRun/litesra/SRR/SRR096/$i/*; done

      This will download the specific SRA files that are listed in the file SRR_2_download

      Thanks
      --
      Muthu

      Comment

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