Hello,
I have several individuals sequenced with Illumina and I want to make variant calling.
Each individuals was sequenced in 3 lanes.
I was wondering if the lane information (stored in RG) is important for the GATK workflow or not ?
This is because I could used the merged file of all lanes for mapping instead of doing 3 mappings because I have 3 lanes, add the lane info and then merge.
Thanks !
Muriel
I have several individuals sequenced with Illumina and I want to make variant calling.
Each individuals was sequenced in 3 lanes.
I was wondering if the lane information (stored in RG) is important for the GATK workflow or not ?
This is because I could used the merged file of all lanes for mapping instead of doing 3 mappings because I have 3 lanes, add the lane info and then merge.
Thanks !
Muriel
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