1. Design: Does ChIP-seq need biological replicates? Is control sample necessary?
2. DNA shearing: If doing ChIP-chip, DNA fragments usually range from 500-600 bp. But when illumina make libraries, they start from DNA with ~200 bp . Do we need to shear DNA to ~200bp during ChIP?
2. DNA shearing: If doing ChIP-chip, DNA fragments usually range from 500-600 bp. But when illumina make libraries, they start from DNA with ~200 bp . Do we need to shear DNA to ~200bp during ChIP?
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