I've processed 8 pacbio Cells corresponding to 3 different IsoSeq libraries with the pbtranscript.py pipeline.
In the classify step of the procedure about 20% of the films (ZMW) do not produce a read of insert (RoI).
When I check the number of reads per film for reads giving and not giving RoIs I get the following result.
Which shows that films with 2 to 5 reads do not produce RoI or produce much less RoIs than other films.
Any idea why?
In the classify step of the procedure about 20% of the films (ZMW) do not produce a read of insert (RoI).
When I check the number of reads per film for reads giving and not giving RoIs I get the following result.
Which shows that films with 2 to 5 reads do not produce RoI or produce much less RoIs than other films.
Any idea why?
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