Why the pacbio isoseq data used for RNA analysis is much more than illumina data?

[huan]

As we all know, for the human RNA-seq analysis, 4-6G clean data from illumina platform is enough. But when I use pacbio's ISO-seq pipline, the subread data is more than 10 times the amount of illumina data. So what causes the difference between the amout of data from this two platform?

Thanks a lot for any reply!

[bowhan]

pacBio has less # of reads than illumina does, but each read is much longer.
And barcoding is more common on illumina platforms. Based on your number (4-6G), you are probably talking about barcoded library.