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Does anyone know of a quicker alternative to PCR/agarose gel when QCing bisulphite conversion in large numbers of samples?
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What assay do you want or run with the converted sample? -
EPIC arraysComment
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I'm mostly posting to also follow this thread since i've also struggled with this.
I've tried the Qubit RNA kit, the zymo bisulfite converted control and just ballparking it based on the expected recovery %. Honestly, qPCR setup might be the best high throughput method for quantitation for you but it didn't quite fulfill all of my projects requirementsComment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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