java -Djava.library.path=/home/Simulation/revision/bbmap/jni/ -ea -Xmx101g -cp /home/Simulation/revision/bbmap/current/ align2.BBMap build=1 overwrite=true fastareadlen=500 ref=/home/Simulation/real_genomes/Mmul_new/Mmul_long.fa nodisk in=/home/Simulation/revision/realGenomes/100SE/Mmul/fastq/Mmul.100SE.rep1.fastq.gz unpigz=t maxindel=200000 local=t threads=1 ambiguous=best out=/home/Simulation/revision/realGenomes/100SE/Mmul/bams/self_Mmul.100SE.rep1.bam pigz=t showprogress=1000000 sam=1.3 nhtag=t nmtag=t statsfile=/home/Simulation/revision/realGenomes/100SE/Mmul/bams/self_Mmul.100SE.rep1.stats -Xmx101g Executing align2.BBMap [build=1, overwrite=true, fastareadlen=500, ref=/home/Simulation/real_genomes/Mmul_new/Mmul_long.fa, nodisk, in=/home/Simulation/revision/realGenomes/100SE/Mmul/fastq/Mmul.100SE.rep1.fastq.gz, unpigz=t, maxindel=200000, local=t, threads=1, ambiguous=best, out=/home/Simulation/revision/realGenomes/100SE/Mmul/bams/self_Mmul.100SE.rep1.bam, pigz=t, showprogress=1000000, sam=1.3, nhtag=t, nmtag=t, statsfile=/home/Simulation/revision/realGenomes/100SE/Mmul/bams/self_Mmul.100SE.rep1.stats, -Xmx101g] Version 38.02 [build=1, overwrite=true, fastareadlen=500, ref=/home/Simulation/real_genomes/Mmul_new/Mmul_long.fa, nodisk, in=/home/Simulation/revision/realGenomes/100SE/Mmul/fastq/Mmul.100SE.rep1.fastq.gz, unpigz=t, maxindel=200000, local=t, threads=1, ambiguous=best, out=/home/Simulation/revision/realGenomes/100SE/Mmul/bams/self_Mmul.100SE.rep1.bam, pigz=t, showprogress=1000000, sam=1.3, nhtag=t, nmtag=t, statsfile=/home/Simulation/revision/realGenomes/100SE/Mmul/bams/self_Mmul.100SE.rep1.stats, -Xmx101g] Set threads to 1 Retaining first best site only for ambiguous mappings. Executing dna.FastaToChromArrays2 [/home/Simulation/real_genomes/Mmul_new/Mmul_long.fa, 1, writeinthread=false, genscaffoldinfo=true, retain, waitforwriting=false, gz=true, maxlen=536670912, writechroms=false, minscaf=1, midpad=300, startpad=8000, stoppad=8000, nodisk=true] Set genScaffoldInfo=true Set genome to 1 Loaded Reference: 0.004 seconds. Loading index for chunk 1-7, build 1 Indexing threads started for block 0-3 Indexing threads finished for block 0-3 Indexing threads started for block 4-7 Indexing threads finished for block 4-7 Generated Index: 512.669 seconds. Analyzed Index: 17.231 seconds. Found samtools 1.8 Started output stream: 0.151 seconds. Cleared Memory: 4.581 seconds. Processing reads in single-ended mode. Started read stream. Started 1 mapping thread. Exception in thread "Thread-12" java.lang.AssertionError at align2.BBIndex.extendScore(BBIndex.java:2612) at align2.BBIndex.slowWalk3(BBIndex.java:1389) at align2.BBIndex.find(BBIndex.java:777) at align2.BBIndex.find(BBIndex.java:623) at align2.BBIndex.findAdvanced(BBIndex.java:400) at align2.AbstractMapThread.quickMap(AbstractMapThread.java:750) at align2.BBMapThread.processRead(BBMapThread.java:408) at align2.AbstractMapThread.run(AbstractMapThread.java:508) Detecting finished threads: 0 ************************************************************************** Warning! 1 mapping thread did not terminate normally. Check the error log; the output may be corrupt or incomplete. Please submit the full stderr output as a bug report, not just this message. ************************************************************************** ------------------ Results ------------------ Genome: 1 Key Length: 13 Max Indel: 200000 Minimum Score Ratio: 0.56 Mapping Mode: normal Exception in thread "main" java.lang.AssertionError: The number of reads out does not add up to the number of reads in. This may indicate that a mapping thread crashed. If you submit a bug report, include the entire console output, not just this error message. 48746+48232+0+503+0 = 97481 != 97482 at align2.AbstractMapper.printOutputStats(AbstractMapper.java:1924) at align2.AbstractMapper.printOutput(AbstractMapper.java:1053) at align2.BBMap.testSpeed(BBMap.java:499) at align2.BBMap.main(BBMap.java:35)