Bismark report for: BSR1_1_1.fq.trimmed.paired1 and BSR1_1_1.fq.trimmed.paired2 Bowtie was run against the bisulfite genome of /home/bioinformatics2/data/bismark_index/ with the specified options: -q --phred64-quals -n 2 -l 28 -e 100 -k 2 --best --minins 50 --maxins 550 --chunkmbs 512 Option '--directional' specified: alignments to complementary strands will be ignored Final Alignment report ====================== Number of paired-end alignments with a unique best hit: 56819646 Mapping efficiency: 63.9% Sequences with no alignments under any condition: 7794380 Sequences did not map uniquely: 24315299 Number of sequences with unique best (first) alignment came from the bowtie output: CT/GA/CT: 28587181 ((converted) top strand) GA/CT/GA: 0 (complementary to (converted) top strand) GA/CT/CT: 0 (complementary to (converted) bottom strand) CT/GA/GA: 28232465 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 16564 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2222006259 Total methylated C's in CpG context: 321380895 Total methylated C's in CHG context: 248390737 Total methylated C's in CHH context: 20423223 Total C to T conversions in CpG context: 95730361 Total C to T conversions in CHG context: 135462511 Total C to T conversions in CHH context: 1400618532 C methylated in CpG context: 77.0% C methylated in CHG context: 64.7% C methylated in CHH context: 1.4%