COMMAND LINE: skewer -x AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG -y AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT -t 8 SRR330569_1.fastq SRR330569_2.fastq Input file: SRR330569_1.fastq Paired file: SRR330569_2.fastq trimmed: SRR330569_1.trimmed-Q0L18-pair1.fastq, SRR330569_1.trimmed-Q0L18-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ (auto detected) -- number of concurrent threads (-t): 8 Tue Feb 25 11:32:32 2014 >> started Tue Feb 25 11:34:14 2014 >> done (102.664s) 27005344 read pairs processed; of these: 1058 ( 0.00%) short read pairs filtered out after trimming by size control 924 ( 0.00%) empty read pairs filtered out after trimming by size control 27003362 (99.99%) read pairs available; of these: 11959533 (44.29%) trimmed read pairs available after processing 15043829 (55.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 116 0.00% 19 11780 0.04% 20 447 0.00% 21 209 0.00% 22 287 0.00% 23 203 0.00% 24 281 0.00% 25 462 0.00% 26 772 0.00% 27 2371 0.01% 28 1778 0.01% 29 2318 0.01% 30 2954 0.01% 31 3831 0.01% 32 3801 0.01% 33 3945 0.01% 34 4916 0.02% 35 6693 0.02% 36 8381 0.03% 37 10210 0.04% 38 11703 0.04% 39 13772 0.05% 40 15256 0.06% 41 17228 0.06% 42 18662 0.07% 43 20341 0.08% 44 22273 0.08% 45 24510 0.09% 46 27515 0.10% 47 30597 0.11% 48 32814 0.12% 49 35568 0.13% 50 39412 0.15% 51 44386 0.16% 52 46936 0.17% 53 50169 0.19% 54 53504 0.20% 55 57380 0.21% 56 63113 0.23% 57 67669 0.25% 58 74728 0.28% 59 78066 0.29% 60 85139 0.32% 61 92738 0.34% 62 99827 0.37% 63 108350 0.40% 64 114629 0.42% 65 122031 0.45% 66 130659 0.48% 67 141540 0.52% 68 147373 0.55% 69 161523 0.60% 70 167153 0.62% 71 183922 0.68% 72 199042 0.74% 73 212157 0.79% 74 211781 0.78% 75 219951 0.81% 76 225731 0.84% 77 245783 0.91% 78 259922 0.96% 79 274157 1.02% 80 280639 1.04% 81 290088 1.07% 82 295182 1.09% 83 303355 1.12% 84 317816 1.18% 85 329503 1.22% 86 343732 1.27% 87 339552 1.26% 88 351034 1.30% 89 362213 1.34% 90 360482 1.33% 91 369942 1.37% 92 392152 1.45% 93 412609 1.53% 94 398153 1.47% 95 439008 1.63% 96 407245 1.51% 97 415674 1.54% 98 405396 1.50% 99 407933 1.51% 100 423060 1.57% 101 15043829 55.71%