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Sticky: Refurbished Hiseq 4000, Nextseq 500, Hiseq 2500v4 & Miseq DNA Sequencers <Sponsored> IET	06-27-2019 07:34 AM by ppssvv	1	9,253

Versus v4 chemistry for whole genome bisulfite sequencing tartarus97	Today 01:53 AM by tartarus97	0	42
Hiscan - iScan control software aco0ob	Today 12:15 AM by ppssvv	1	493
NextSeq 1000 and 2000 cluster density and number of copies Dornfield	02-20-2020 03:40 AM by Dornfield	0	252
Overlapping paired-end reads for rare mutation detection maxz411	02-19-2020 11:18 PM by SNPsaurus	1	225
High proportion of PolyT reads days369	02-19-2020 01:20 PM by BlakeGriffin	1	2,197
SR Rapid V2 compared to SR V3/V4 flow cells arikogran	02-18-2020 01:08 PM by misterc	1	222
Gel breaker tube alternatives ES5063	02-17-2020 09:51 AM by ES5063	0	268
1/5 Nextera Flex number of cycles mdegraaf	02-12-2020 11:02 AM by mdegraaf	0	276
Problems with MiniSeq sequencing Noveline	02-06-2020 07:42 AM by Noveline	2	287
NovaSeq reagent cost in 2020 weigrc	02-04-2020 06:27 PM by weigrc	0	253
Change standard index sequence in sample sheet for Miseq Tom2013	02-04-2020 04:28 PM by GenoMax	3	327
Illumina emlas	02-03-2020 05:11 AM by emlas	0	221
10x genomics 3'RNA-seq dual indexing martin2801	01-27-2020 06:33 AM by martin2801	3	650
NGS sequencing and capture probes ChiaraGen	01-15-2020 02:00 AM by fennycruz	4	1,445
Would you sequence an RNA-Seq library with this amount of adapter dimer? 0880484929	01-09-2020 02:02 AM by lexogen	1	672
3' Profile Bias using Illumina Next Seq aferng	01-06-2020 03:16 PM by aferng	0	398
Ultrapure ligase from Enzymatics Aero	01-03-2020 10:18 PM by Purification2013	6	4,750
MiSeq - issue with index and read 2 (Low Q30) Ross_Mcf	12-18-2019 07:28 PM by Dallin1232	6	1,363
NextSeq550_LowClusterDensity_Low%PF? 7891_ZA	12-12-2019 08:29 AM by Johnwang	1	599
10x genomics and 'paired end' reads zauhar	12-09-2019 03:05 AM by SeqCharlotte	7	4,297
Exact duplicate reads/readnames/quality/tiles in Novaseq FASTQs ECO	12-05-2019 02:53 PM by saimonara	6	1,910
Error in mvnX when running processSamples.R of OncoCNV mwarrier	12-05-2019 01:52 PM by mwarrier	0	508
Unexpected power failure during run CPHSEQer	12-03-2019 09:28 AM by CPHSEQer	4	1,382
Can I use default sequencing primers for Miseq? Tom2013	12-02-2019 12:47 PM by Tom2013	3	771
Allele Frequency Reproducibility: An interesting observation AmitChaurasia	11-28-2019 08:04 PM by AmitChaurasia	2	856
bcl2fastq on windows? jdb	11-22-2019 07:00 AM by LarsB	5	3,569
Freezing Miseq cartridges at -80°C? minio.cz	11-14-2019 06:05 AM by ppssvv	1	914
Low data output from questionable sequencing facility samd	11-06-2019 06:45 PM by samd	18	1,513
NovaSeq 6000 chaase	11-06-2019 06:50 AM by chaase	0	771
QIAseq FX DNA Library Kit adapters for trimming ciemanek	10-29-2019 04:44 AM by ciemanek	7	1,589

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