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Sticky: Refurbished Hiseq 4000, Nextseq 500, Hiseq 2500v4 & Miseq DNA Sequencers <Sponsored> IET	06-27-2019 07:34 AM by ppssvv	1	7,969

10x genomics and 'paired end' reads zauhar	Today 03:05 AM by SeqCharlotte	7	3,454
Exact duplicate reads/readnames/quality/tiles in Novaseq FASTQs ECO	12-05-2019 02:53 PM by saimonara	6	1,341
Error in mvnX when running processSamples.R of OncoCNV mwarrier	12-05-2019 01:52 PM by mwarrier	0	76
Unexpected power failure during run CPHSEQer	12-03-2019 09:28 AM by CPHSEQer	4	803
Can I use default sequencing primers for Miseq? Tom2013	12-02-2019 12:47 PM by Tom2013	3	286
Allele Frequency Reproducibility: An interesting observation AmitChaurasia	11-28-2019 08:04 PM by AmitChaurasia	2	390
MiSeq - issue with index and read 2 (Low Q30) Ross_Mcf	11-25-2019 09:46 AM by itstrieu	5	399
bcl2fastq on windows? jdb	11-22-2019 07:00 AM by LarsB	5	3,017
Freezing Miseq cartridges at -80°C? minio.cz	11-14-2019 06:05 AM by ppssvv	1	395
Low data output from questionable sequencing facility samd	11-06-2019 06:45 PM by samd	18	906
NovaSeq 6000 chaase	11-06-2019 06:50 AM by chaase	0	336
QIAseq FX DNA Library Kit adapters for trimming ciemanek	10-29-2019 04:44 AM by ciemanek	7	905
NEBNext adaptors clustering failed bsvega	10-25-2019 02:22 AM by bsvega	14	2,213
MiSeq tolerated library size distribution ES5063	10-24-2019 03:10 AM by ES5063	6	558
NovaSeq metatranscriptomics furor	10-23-2019 05:24 AM by GenoMax	2	827
qPCR standard variability with Kapa kit kgoglin	10-16-2019 07:41 AM by kgoglin	2	748
NextSeq underclustering acockburn	10-16-2019 07:20 AM by acockburn	0	472
am I overclustered -- MiSeq wei210	10-12-2019 07:21 PM by wei210	2	575
Kapa lib quant question wei210	10-10-2019 06:18 AM by wei210	2	605
Negative controls and index hopping Illumina HiSeq - Metagenomics robertsr	10-10-2019 05:25 AM by kmcarr	2	656
Negative controls and index hopping Illumina HiSeq - Metagenomics robertsr	10-08-2019 06:16 PM by robertsr	0	402
Question for Illumina Miseq/Nextseq owners Tim2	10-08-2019 01:25 PM by Tim2	2	737
Why is the ME-rev oligo on Tn5 phosphorylated? Adem80	10-01-2019 10:56 AM by SCIL2019	11	2,019
Artemis paired end illumina short read mapping won't display any "duplicate" reads leitouran	09-30-2019 02:37 PM by leitouran	0	560
Changing index read length for Miseq run huatrongkhang	09-30-2019 06:34 AM by pmiguel	2	795
Consistent low MiSeq cluster density and PhiX alignment with high quality reads (PF%) mamor	09-23-2019 11:19 AM by thermophile	14	2,732
How many tiles does an iSeq have? szy0931	09-23-2019 09:12 AM by Grandol1	3	1,110
Miniseq problem help CALseq1	09-16-2019 11:29 AM by CALseq1	0	699
Single end read WGBS Notorious ATG	09-13-2019 06:52 AM by fkrueger	1	676
Non-Barcoded reads. Why? lncRNA	09-01-2019 09:26 AM by lncRNA	0	1,015

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