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Sticky: Refurbished Hiseq 4000, Nextseq 500, Hiseq 2500v4 & Miseq DNA Sequencers <Sponsored> IET	06-27-2019 06:34 AM by ppssvv	1	21,556

Why there are some nonsense repetitive reads shown in the sequencing result fasta? btphzxc	05-02-2022 11:06 AM by btphzxc	0	683
quality score issues dubravka.pezic@labgeni.us	04-29-2022 07:01 AM by dubravka.pezic@labgeni.us	0	280
Miniseq run issues tired_of_overclustering	04-18-2022 01:53 PM by tired_of_overclustering	0	395
Low data output from questionable sequencing facility samd	04-13-2022 04:23 AM by ddabiri	19	4,720
specific compost/soil metagenomes causing underclustering of whole run? jov14	04-11-2022 04:38 AM by jov14	0	330
Liquid Handling Instruments ( 1 2) santorini	04-04-2022 11:50 AM by WhiteSeal	20	12,801
Have I understood the ASCII-FastQ Offset 33 concept properly? Lostin	03-30-2022 03:53 AM by Lostin	0	551
Informatica Admin Console won't come up rakheehkr	03-29-2022 04:34 AM by rakheehkr	0	474
Custom Library prep kit for UDI Vita9285	03-20-2022 04:41 AM by Vita9285	0	574
Mutation carried on only one strand? ShivaNGS	03-13-2022 05:16 PM by ShivaNGS	0	607
Problem with indexes/demultiplexing pfv225	02-20-2022 04:14 AM by GenoMax	1	926
what is the sequence of Illumina NovaSeq sequencing primers? seqman111	02-15-2022 02:06 PM by seqman111	0	740
MiSeq maintenance wash issue DMartin	02-04-2022 04:23 PM by RadishBlood	2	3,144
staggered custom sequencing primers to increase diversity IonVsIllumina	01-31-2022 12:26 PM by gurtecho	1	1,136
Run status "Uploading" lasts for 10 hours (NextSeq500) Vita9285	01-20-2022 05:42 AM by Vita9285	2	1,075
Consistent low MiSeq cluster density and PhiX alignment with high quality reads (PF%) mamor	01-05-2022 08:39 AM by razzaowl	15	6,737
MiSeq Micro kit issue, no data except cluster density KyleWolf	12-15-2021 08:32 AM by pmiguel	6	2,179
MiSeq problem with .locs files lubobal	12-12-2021 05:01 AM by GenoMax	1	1,305
MiSeq - issue with index and read 2 (Low Q30) Ross_Mcf	11-28-2021 08:07 PM by hirohaha	7	5,346
Weird Base Distribution in 10X DNA library Drmadscientist	11-24-2021 08:01 PM by Drmadscientist	0	1,482
BCL Convert Performance Gains thefabnab	11-02-2021 09:11 PM by j13kagrf	3	4,909
cDNA amplyfication in smart-seq2 Amberlycole	10-28-2021 04:20 AM by Amberlycole	0	2,463
Miseq LaneQC and Flow Cell failed - but Data By Cycle looks correct XmaX	10-15-2021 01:09 AM by XmaX	0	1,703
p5/p7 flow cell bound oligo question nutterson	09-24-2021 04:19 PM by luc	12	14,197
Do I need to use a library preparation kit with MiSeq? XmaX	09-24-2021 04:14 PM by luc	1	2,115
What indexing should I use? XmaX	09-22-2021 03:02 PM by BioDynami	1	2,214
P5/P7 Flowcell Oligos jlove	09-20-2021 07:47 PM by darklight	6	6,789
Novaseq 150PE data output mpl	09-17-2021 05:43 AM by mpl	2	2,363
timeout of 60.000 seconds exceeded while waiting for FPGA command "ZDACR" to complete sandra.rei	09-02-2021 05:16 AM by misterc	1	3,062
LRM software with EMP primers cement_head	09-01-2021 10:25 AM by cement_head	0	2,111

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