Sample Prep / Library Generation

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Sample Prep / Library Generation

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Make your own AMPure XP, cheaply jwfoley	Today 11:40 AM by teuthbrush	15	11,056
Inefficient ATAC-seq library prep amplification lapensee	12-01-2019 08:10 PM by Meyana	18	3,239
How to pool ATAC-seq libraries in equimolar concentrations? ECorrales	11-22-2019 03:39 PM by ECorrales	4	364
Mixing cDNA samples from Ovation RNA‐Seq System V2 of NuGEN with Nextera Dual Indexes seq_uestion	11-19-2019 08:34 AM by luc	1	249
Ribozero Bacteria discontinued - alternatives? Genetic Librarian	11-12-2019 06:45 AM by MU Core	16	4,274
PCR amplicon library - DNA smearing microgirl123	11-07-2019 05:24 PM by luc	1	332
discrepancy in miseq runs due to sample prep protocol? samd	11-07-2019 07:33 AM by kmcarr	2	327
EpMotion - NEBnext ultra program acockburn	11-01-2019 02:30 AM by macgenome	1	1,817
(2x) Final Bead Clean-Up cement_head	10-30-2019 04:34 PM by luc	2	489
high rRNA after poly(A) selection nboom	10-25-2019 02:56 AM by Michael.Ante	1	644
ATACseq library prep without commercial bought kit lineras	10-20-2019 09:47 PM by Meyana	2	847
Fragment Analyzer reliability issues LVAndrews	10-18-2019 03:42 PM by LVAndrews	6	866
Is this library overamplification? Is this problematic? Carcharodon	10-18-2019 01:05 PM by JackieM	2	677
smaller RNA gene panel from TST170? ps25	10-18-2019 06:59 AM by ps25	0	334
Neutralize Tagment Buffer (NT) michael_liu	10-18-2019 02:46 AM by Adz	5	6,619
RNA purification, DNA contamination, and small RNA sizing profiles kumard	10-08-2019 06:18 PM by luc	10	820
n00b Smart-seq2 questions chilipep	10-08-2019 03:47 PM by cmbetts	3	511
home made EZ-Tn5-Nextera adapter complex? silin284	10-01-2019 11:02 AM by SCIL2019	1	2,524
RNA extraction and DNase treatment rubyryan	09-27-2019 01:42 PM by kumard	18	12,922
Tapestation imaging provirus	09-25-2019 03:48 AM by provirus	0	552
Nextera Kit -- DNA fragments too small ( 1 2) arilindsey	09-14-2019 03:35 PM by SCIL2019	20	7,226
Library GC QC before sequencing BexMultiplex	09-11-2019 05:19 PM by luc	1	780
Why is it commonly recommended to Speed-Vac without heat? Carcharodon	09-11-2019 05:14 PM by luc	5	957
More than half of Smart-seq2 data is TSO,ISPCR concatemers! Kujin Kwon	09-05-2019 10:14 AM by luc	1	771
Targeted amplicon bisulfite sequencing library preparation kk123	09-05-2019 04:27 AM by kk123	4	1,982
Suggestions for pre-Library Prep QC on FFPE samples kwaraska	09-04-2019 02:06 PM by itstrieu	1	727
Dimerator adaptor removal by Diacarta pkianian	09-03-2019 04:29 PM by luc	1	652
Roche SeqCap protocol problems eriech	09-02-2019 10:41 PM by eriech	0	577
Agilent SS All Exon V7 kit baru	08-28-2019 06:30 PM by itstrieu	5	1,319
Homemade AMPure XP has some problems luolipang	08-28-2019 01:02 AM by luolipang	0	811

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