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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,195
Posts: 9,880
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2,195 | 9,880 |
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 6
Posts: 10
Last Post:
bam file missing barcode and UMI info
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6 | 10 |
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 278
Posts: 991
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278 | 991 |
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De novo discovery
Wandering without a reference? Post here
Topics: 341
Posts: 1,700
Last Post:
infering isoforms in merged transcriptome
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341 | 1,700 |
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273
Posts: 852
Last Post:
Impact of biomass on shotgun sequencing
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273 | 852 |
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357
Posts: 1,578
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357 | 1,578 |
12-09-2023, 09:17 PM
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,866
Posts: 11,414
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2,866 | 11,414 |
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42
Posts: 107
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42 | 107 |
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Join us for our insightful SEQanswers webinar "Harnessing NGS in Cancer Research." This session will feature a panel of scientists discussing...03-18-2024, 08:22 AM
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM -
The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...03-08-2024, 10:41 AM