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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,195
Posts: 9,880
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2,195 | 9,880 |
04-15-2024, 07:08 PM
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 6
Posts: 10
Last Post:
bam file missing barcode and UMI info
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6 | 10 |
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 278
Posts: 991
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278 | 991 |
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De novo discovery
Wandering without a reference? Post here
Topics: 341
Posts: 1,700
Last Post:
infering isoforms in merged transcriptome
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341 | 1,700 |
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273
Posts: 852
Last Post:
Impact of biomass on shotgun sequencing
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273 | 852 |
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357
Posts: 1,578
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357 | 1,578 |
12-09-2023, 09:17 PM
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,866
Posts: 11,414
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2,866 | 11,414 |
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42
Posts: 107
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42 | 107 |
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Genomics technologies have significantly enhanced our comprehension of the complex immune system. Our upcoming webinar explores innovative applications...05-01-2024, 06:50 AM
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Join us for our insightful SEQanswers webinar "Harnessing NGS in Cancer Research." This session will feature a panel of scientists discussing...03-18-2024, 08:22 AM
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM