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Sticky: Refurbished Hiseq 4000, Nextseq 500, Hiseq 2500v4 & Miseq DNA Sequencers <Sponsored> IET	06-27-2019 07:34 AM by ppssvv	1	9,264

Would you sequence an RNA-Seq library with this amount of adapter dimer? 0880484929	01-09-2020 02:02 AM by lexogen	1	677
18s V4 region in Illumina MiSeq Amplicon 18sSpace	03-26-2014 06:41 AM by cement_head	6	3,978
Restriction enzymes for genomic DNA library prep (e.g. NlaIII) 454newbie	07-07-2011 08:14 PM by 454newbie	7	5,428
NextSeq550_LowClusterDensity_Low%PF? 7891_ZA	12-12-2019 08:29 AM by Johnwang	1	606
Wierd base calling on MiniSeq run AAG_GGA	08-21-2018 06:30 AM by AAG_GGA	0	1,370
Aptamer sequencing on MiSeq AAR	10-08-2015 08:41 PM by BiochemPhD	1	1,699
How much phiX to spike in for ddRADseq library on HiSeq? AaronReedy	05-10-2016 05:23 AM by Jean-Rene	7	2,878
Would it be OK if the Read 1 and Read 2 Seq Primer are identical for Myseq? aaron_tao	06-03-2015 09:08 AM by aaron_tao	17	2,979
Which coverage for resequencing genome abastkowska	02-26-2015 01:58 AM by abastkowska	2	1,351
Custom Sequencing Primers abbecker	01-11-2018 02:34 AM by abbecker	0	951
Sequencing using P7 adaptor abbecker	01-29-2018 03:34 PM by jwfoley	1	1,151
Can I use DESeq and edgeR for mixed ANOVA abebe	07-13-2017 05:45 AM by Simon Anders	18	13,693
paired-end question abelhj	12-30-2009 02:01 PM by ECO	1	2,517
ChIPseq wig file with the input subtracted from ChIP sample abmmki	08-02-2012 06:51 PM by captainentropy	1	2,314
Miseq Paired End read quality much poorer! Why?? abyss	04-29-2013 09:21 AM by GenoMax	11	8,552
Low Cluster Densities in MiSEQ?? Possible explanations??? ( 1 2) abyss	07-08-2015 11:42 AM by Jessica_L	30	24,562
MiSeq 16S reads (dumb question) Accentor	09-23-2013 08:06 AM by Accentor	2	2,853
Base distribution plot split issue acdan	12-15-2017 01:45 PM by Andres_Ribone	13	2,396
agarose instead of Bio-rad low range acdan	11-18-2015 07:11 PM by acdan	0	964
Ladder-like bioanalyzer trace on supposed 150bp DNA sample library Ace5858	03-31-2012 02:39 PM by pmiguel	1	2,930
Sequencing NYC Subway bacteria???? Ace5858	08-15-2013 08:47 AM by Ace5858	0	1,274
Confusion about single end vs paired end read output on Illumina HiSeq acidcoated	03-19-2015 09:56 AM by GenoMax	1	3,247
Hiscan - iScan control software aco0ob	Yesterday 12:15 AM by ppssvv	1	551
Question about SAMstrt Spike-in RNA acoada	12-26-2013 04:32 AM by acoada	2	1,876
Amplification free library preparation AcoBetalow	12-11-2014 07:37 AM by AcoBetalow	3	1,788
NextSeq underclustering acockburn	10-16-2019 07:20 AM by acockburn	0	902
Opinions/Experiences with targeted RNA-Seq? acornwell	06-01-2015 08:06 AM by acornwell	0	1,182
TruSeq Library Pooling - Balanced Indexes? acpetterson	01-25-2013 05:55 AM by pmiguel	6	4,994
Regular RTA Stalls with HiSeq 2000 acunning	05-03-2013 07:54 AM by pmiguel	5	30,503
Mate pair de novo assembly AdaLovelace	05-17-2016 05:43 AM by Markiyan	2	2,027

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