Sample Prep / Library Generation

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Sample Prep / Library Generation

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"random" enzymatic DNA fragmentation - how does it work? luc	Yesterday 09:14 AM by luc	0	175
Crispr library which gene? frymor	01-20-2019 12:18 AM by frymor	0	109
Ribozero Bacteria discontinued - alternatives? Genetic Librarian	01-18-2019 08:31 AM by siTOOLs	4	793
Single-stranded DNA library preparation? Iikki	01-17-2019 07:12 AM by BioDynami	1	140
Direct Bacterial Colony Sequencing with the Nextera DNA Flex sdirienzi	01-14-2019 09:11 AM by sdirienzi	0	132
End Repair + A-Tailing - how do they do it? Ingeneious	01-14-2019 08:51 AM by luc	15	12,850
8 nt Universal Blockers (blocking oligos) for 10 bp index? aengstrom	01-12-2019 01:08 PM by aengstrom	0	165
NExtflex adapters for Truseq rnaquestions	01-11-2019 03:46 PM by luc	3	315
Low-concentration, AT rich genome sequencing rflrob	01-10-2019 01:42 PM by rflrob	0	152
Correct Ratio for two-sided PCR-cleanup with Ampure XP beads bheida	01-10-2019 02:01 AM by JakobHedegaard	2	455
Library degrading after prep with Ovation Ultralow V2 robicha7	01-04-2019 07:01 AM by robicha7	5	996
Ligation inefficiencies with NEB Sabre_seq	01-03-2019 04:11 PM by nucacidhunter	1	238
ScriptSeq v2 135base-peak JasperGeh	12-17-2018 11:31 PM by luc	4	381
RNase treatment before NGS metagenomic community profiling? Yea or nay? drdoodlebug	12-15-2018 02:33 AM by nucacidhunter	1	262
Inefficient ATAC-seq library prep amplification lapensee	12-13-2018 04:38 PM by Meyana	3	669
ATACseq - large nucleosome peaks pennypeverell	12-12-2018 03:56 AM by Grg91	7	1,507
Is my ATACseq library okay? coralxanpu	12-11-2018 01:01 AM by Grg91	6	1,546
Circular chromatin conformation capture carlos aylwin	11-27-2018 11:17 AM by carlos aylwin	0	375
Large DNA fragments after ChIP on histone modifications MGineste	11-26-2018 04:19 PM by KB*	15	18,941
UV Transilluminator? Colaptes	11-26-2018 07:40 AM by pmiguel	4	546
Agilent Bioanalyzer: Unexpected signal in 5S region juelraja	11-22-2018 01:06 PM by Seqasaurus	17	8,030
Adaptor dimer contamination paolo.kunder	11-20-2018 11:07 AM by liuqy	5	5,773
Smart-seq2 sample prep questions PrimerBuffalo	11-19-2018 09:08 AM by Simone78	3	1,574
Targeted amplicon bisulfite sequencing library preparation kk123	11-19-2018 03:16 AM by kk123	2	504
Resuspension Buffer (RSB) added to RNA samples in Truseq mRNA Protocol GenomeM	11-19-2018 01:19 AM by GSviral	4	587
Mechanism of ion torrent Adapter ligation, Use with KAPA Hyper Plus Library Kit? Atzin	11-14-2018 03:53 AM by torben	1	594
Library degradation of size select? robicha7	11-13-2018 08:57 AM by robicha7	0	357
DNA cleanup kits smoics	11-07-2018 11:56 AM by teuthbrush	2	744
qubit standards left at RT overnight smoics	11-06-2018 06:25 AM by Genetic Librarian	3	663
BluePippin vs PippinPrep cement_head	11-01-2018 05:20 AM by kmcarr	4	631

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