Techniques and protocol discussions on sample preparation, library generation, methods and ideas

Variant discovery in previously sequenced genomes/regions

Wandering without a reference? Post here

Ever wonder what's growing in that hot spring or glacier?

Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.

Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)

Discuss issues unique to clinical sequencing.