Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

Open discussion forum for Open Genomics Engine contributors and users

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Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)

Service-based whole human genome sequencing with probe ligation chemistry.

True Single Molecule Sequencing

Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)

Integrated electronic detection of protons released upon base incorporation. (Life Technologies)

Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures

"Open Source" instrument and sequencing by ligation chemistry (Danaher Motion-Dover)

Automated dye terminator ("Sanger") chemistries

Sequencing by Oligo Ligation/Detection (Life Technologies)

Sequencing technologies without a commercially released platform (Oxford Nanopore, Halcyon Molecular, etc.)

Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.

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Techniques and protocol discussions on sample preparation, library generation, methods and ideas

Variant discovery in previously sequenced genomes/regions

Wandering without a reference? Post here

Ever wonder what's growing in that hot spring or glacier?

Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.

Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)

Discuss issues unique to clinical sequencing.

Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.

Announcements regarding SEQanswers

Please post all feedback or suggestions about the site in this forum.

Discussions about SEQwiki. Problems, suggestions, offers to help...

Planning and feedback subforum for planning sequencing webinar episodes