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News
News about the latest advances and discoveries in sequencing and related technologies.
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Dealing with customer samples, data, and the challenges that come with both?
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127 577
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Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
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2,615 7,818
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Upcoming and on-demand webinars by SEQanswers and its partners.
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Last Post: On-Demand Webinars
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seqadmin
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Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
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41,653 42,332
Sequencing Technologies/Companies
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 53 Posts: 165
53 165
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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2 5
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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3,057 16,723
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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392 2,174
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
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118 598
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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385 2,176
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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76 278
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79 480
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A place for discussion of older forums and sequencing technologies.
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Last Post: Rapid Libraries
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pmiguel
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dsturgill
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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fchatonnet
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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278 991
De novo discovery
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342 1,701
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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273 852
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
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357 1,578
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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2,872 11,430
Clinical Sequencing
Discuss issues unique to clinical sequencing.
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42 107
Personalized Genomics
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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    Genetic Variation in Immunogenetics and Antibody Diversity



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM
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    Choosing Between NGS and qPCR



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM
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    Non-Coding RNA Research and Technologies




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    Nobel Prize for MicroRNA Discovery
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    10-07-2024, 08:07 AM

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