-
Hi all, I’m pretty new to NGS and library prep. I just completed library prep and WES for a pilot of 12 FFPE DNA samples, and got back Fastq files that...Yesterday, 04:32 PM -
Hi, I want to remove fragments larger than 2500 bp from my cDNA library. Would you recommend a 0.6× or 0.7× bead purification ratio?
...02-19-2025, 11:22 PM -
Dear all,
there are only 3 seats left for the 4th edition of the Physalia Introduction to Machine Learning (ML) with R course, taking place online...02-19-2025, 07:01 AM
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CoCoRV reference file? (WES)
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
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G400 fastq header description?
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Weird 16S custom primer issue on Aviti runs
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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ion torrent barcode sequences
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Single-molecule sequencing via a protein nanopore.
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Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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02-20-2024, 05:21 AM
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Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Automated dye terminator ("Sanger") chemistries
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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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02-18-2025, 03:30 AM
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Machine Learning with R course
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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by irate.pirate
Yesterday, 04:32 PM
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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Reference mapping for scRNA seq data?
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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Impact of biomass on shotgun sequencing
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Sonication conditions on ChIP-seq assay?
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11-27-2024, 12:18 AM
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
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| Personalized Genomics | ||||||||||||
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Personalized Genomics
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-
Hi all, I’m pretty new to NGS and library prep. I just completed library prep and WES for a pilot of 12 FFPE DNA samples, and got back Fastq files that...Yesterday, 04:32 PM
-
Hi, I want to remove fragments larger than 2500 bp from my cDNA library. Would you recommend a 0.6× or 0.7× bead purification ratio?
...02-19-2025, 11:22 PM -
Dear all,
there are only 3 seats left for the 4th edition of the Physalia Introduction to Machine Learning (ML) with R course, taking place online...02-19-2025, 07:01 AM -
Dear all,
We still have a few seats available for our upcoming online course: Gene Set Enrichment Analysis in R/Bioconductor, in March (3-6)....02-19-2025, 06:56 AM -
Good morning,
in the last two 16S runs performed (Miseq tool), in the fastqc of the reads a low quality is noted in the first 20 positions or so,...02-19-2025, 01:27 AM
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Single-cell sequencing offers new opportunities to study diseases at a cellular level. Researchers can now study individual cells...02-04-2025, 10:30 AM
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Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.
Nucleic Acid Quality Control
Preparing for NGS starts with isolating the...02-10-2025, 01:58 PM -
In recent years, precision medicine has become a major focus for researchers and healthcare professionals. This approach offers personalized treatment and wellness plans by utilizing insights from each person's unique biology and lifestyle to deliver more effective care. Its advancement relies on innovative technologies that enable a deeper understanding of individual variability. In a joint documentary with our colleagues at Biocompare, we examined the foundational principles of precision...01-27-2025, 07:46 AM -
Spatial biology is an exciting field that encompasses a wide range of techniques and technologies aimed at mapping the organization and interactions of various biomolecules in their native environments. As this area of research progresses, new tools and methodologies are being introduced, accompanied by efforts to establish benchmarking standards and drive technological innovation.
3D Genomics
While spatial biology often involves studying proteins and RNAs in their...01-01-2025, 07:30 PM