Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

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Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing

Bridged amplification & clustering

Sequencing by Oligo Ligation/Detection

Instrument and chemistry launched by the George Church Lab and Danaher Motion-Dover

True Single Molecule Sequencing

Pacific Biosciences, Ion Torrent, Complete Genomics, etc. Suggest others!

Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.

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Techniques and protocol discussions on sample preparation, library generation, methods and ideas

Variant discovery in previously sequenced genomes/regions

Wandering without a reference? Post here

Ever wonder what's growing in that hot spring or glacier?

Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.

Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)

Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.

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