Bioinformatics Pipeline Development with Nextflow
How to manage your own data analysis pipelines using workflow management systems
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Hi everyone, I have recently sequenced 1 lane of NovaSeq X Plus 10B with a sequencing provider. The library that was sequenced is a multiplex WGS lib...Yesterday, 07:14 PM
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Hi everyone, I understand that the DNA precipitation method is largely and commonly used for genomic DNA. May I check if it is suitable for short and...Yesterday, 07:08 PM
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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
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by Naima96
12-21-2023, 07:13 AM
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Sequencing Resources
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News
News about the latest advances and discoveries in sequencing and related technologies.
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Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
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post-bisulphite conversion QC check
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General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
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Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
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Harnessing NGS in Cancer Research
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Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653
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by Biotechvana
03-27-2023, 11:17 PM
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 51
Posts: 160
Last Post:
Setting up and using MGI platforms
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 1
Posts: 1
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,047
Posts: 16,700
Last Post:
Novaseq 600 very low insert size
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 391
Posts: 2,173
Last Post:
Bioanalyzer indispensable?
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Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 117
Posts: 594
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385
Posts: 2,176
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385 | 2,176 |
by Osingh
02-20-2024, 05:21 AM
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0
Posts: 0
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0
Posts: 0
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76
Posts: 275
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SeqStudio users?
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Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79
Posts: 480
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Archived Forums
A place for discussion of older forums and sequencing technologies.
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Last Post:
Rapid Libraries
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Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
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Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
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Senior Scientist, NGS
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Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
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01-08-2024, 03:56 AM
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,195
Posts: 9,880
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2,195 | 9,880 |
Yesterday, 07:08 PM
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 6
Posts: 10
Last Post:
bam file missing barcode and UMI info
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 278
Posts: 991
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De novo discovery
Wandering without a reference? Post here
Topics: 341
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Last Post:
infering isoforms in merged transcriptome
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273
Posts: 852
Last Post:
Impact of biomass on shotgun sequencing
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357
Posts: 1,578
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357 | 1,578 |
12-09-2023, 09:17 PM
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,866
Posts: 11,414
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42
Posts: 107
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 57
Posts: 290
Last Post:
Bione Genetic Testing for Cancer
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Site Announcements
Announcements regarding SEQanswers
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Heading to ASHG 2023
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Making a table in BBCode does not work
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Bioinformatics Pipeline Development with Nextflow
How to manage your own data analysis pipelines using workflow management systems
...Today, 04:25 AM -
Hi everyone, I have recently sequenced 1 lane of NovaSeq X Plus 10B with a sequencing provider. The library that was sequenced is a multiplex WGS lib...Yesterday, 07:14 PM
-
Hi everyone, I understand that the DNA precipitation method is largely and commonly used for genomic DNA. May I check if it is suitable for short and...Yesterday, 07:08 PM
-
Hi, I've been seeing very short insert sizes on the Novaseq 6000, using 2x150bp whole genome sequencing.
Insert sizes inferred from mapping reads...04-12-2024, 06:29 AM
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Join us for our insightful SEQanswers webinar "Harnessing NGS in Cancer Research." This session will feature a panel of scientists discussing...03-18-2024, 08:22 AM
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM -
The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...03-08-2024, 10:41 AM
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