Hi Guys,
I've got some Illumina sequencing data from genomic bacterial DNA, however I have reason to believe that my original DNA sample may have contained some single stranded DNA (from a phage).
My question is, will this DNA also have been sequenced? What happens to ssDNA during library prep?
I understand that there is a step after nebulization, which 'fills in' single stranded overhangs. Will this also fill in fragments that are totally single stranded? Or does the T4 polymerase need a double stranded section to initiate filling in?
Thanks for your help,
Kerensa.
I've got some Illumina sequencing data from genomic bacterial DNA, however I have reason to believe that my original DNA sample may have contained some single stranded DNA (from a phage).
My question is, will this DNA also have been sequenced? What happens to ssDNA during library prep?
I understand that there is a step after nebulization, which 'fills in' single stranded overhangs. Will this also fill in fragments that are totally single stranded? Or does the T4 polymerase need a double stranded section to initiate filling in?
Thanks for your help,
Kerensa.
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