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Related Articles Metagenomic analyses of viruses in the stool of children with acute flaccid paralysis.
J Virol. 2009 Feb 11;
Authors: Victoria JG, Kapoor A, Li L, Blinkova O, Slikas B, Wang C, Naeem A, Zaidi S, Delwart E
We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with non-polio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of viral capsid-protected nuclease-resistant nucleic acids was followed by DNA sequencing and sequence similarity searches to identify known and new viruses. Limited Sanger sequencing (35-240 subclones per sample) identified an average of 1.4 eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sample. In addition to bacteriophage and plant viruses, we detected known enteric viruses including rotavirus, adenovirus, picobirnavirus, and human enterovirus (HEV) species A-C, as well as numerous other members of the Picornaviridae family including parechovirus, Aichi virus, rhinovirus, and human cardiovirus. The most divergent viral sequences relative to reported viruses included members of a novel Picornaviridae genus and four new viral species (members of the Dicistroviridae, Nodaviridae, Circoviridae families and the Bocavirus genus). Six healthy contacts of AFP cases were similarly analyzed and also contained numerous viruses, particularly HEV-C, including a potentially novel Enterovirus genotype. Determining the prevalence and pathogenicity of the novel genotypes, species, genera, and potential new viral families identified in this study in different demographic groups will require further studies now facilitated by knowledge of their genomes.
PMID: 19211756 [PubMed - as supplied by publisher]
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Related Articles Metagenomic analyses of viruses in the stool of children with acute flaccid paralysis.
J Virol. 2009 Feb 11;
Authors: Victoria JG, Kapoor A, Li L, Blinkova O, Slikas B, Wang C, Naeem A, Zaidi S, Delwart E
We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with non-polio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of viral capsid-protected nuclease-resistant nucleic acids was followed by DNA sequencing and sequence similarity searches to identify known and new viruses. Limited Sanger sequencing (35-240 subclones per sample) identified an average of 1.4 eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sample. In addition to bacteriophage and plant viruses, we detected known enteric viruses including rotavirus, adenovirus, picobirnavirus, and human enterovirus (HEV) species A-C, as well as numerous other members of the Picornaviridae family including parechovirus, Aichi virus, rhinovirus, and human cardiovirus. The most divergent viral sequences relative to reported viruses included members of a novel Picornaviridae genus and four new viral species (members of the Dicistroviridae, Nodaviridae, Circoviridae families and the Bocavirus genus). Six healthy contacts of AFP cases were similarly analyzed and also contained numerous viruses, particularly HEV-C, including a potentially novel Enterovirus genotype. Determining the prevalence and pathogenicity of the novel genotypes, species, genera, and potential new viral families identified in this study in different demographic groups will require further studies now facilitated by knowledge of their genomes.
PMID: 19211756 [PubMed - as supplied by publisher]
More...