Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • PubMed: Metagenomic analyses of viruses in the stool of children with acute flaccid p

    Syndicated from PubMed RSS Feeds

    Related Articles Metagenomic analyses of viruses in the stool of children with acute flaccid paralysis.

    J Virol. 2009 Feb 11;

    Authors: Victoria JG, Kapoor A, Li L, Blinkova O, Slikas B, Wang C, Naeem A, Zaidi S, Delwart E

    We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with non-polio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of viral capsid-protected nuclease-resistant nucleic acids was followed by DNA sequencing and sequence similarity searches to identify known and new viruses. Limited Sanger sequencing (35-240 subclones per sample) identified an average of 1.4 eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sample. In addition to bacteriophage and plant viruses, we detected known enteric viruses including rotavirus, adenovirus, picobirnavirus, and human enterovirus (HEV) species A-C, as well as numerous other members of the Picornaviridae family including parechovirus, Aichi virus, rhinovirus, and human cardiovirus. The most divergent viral sequences relative to reported viruses included members of a novel Picornaviridae genus and four new viral species (members of the Dicistroviridae, Nodaviridae, Circoviridae families and the Bocavirus genus). Six healthy contacts of AFP cases were similarly analyzed and also contained numerous viruses, particularly HEV-C, including a potentially novel Enterovirus genotype. Determining the prevalence and pathogenicity of the novel genotypes, species, genera, and potential new viral families identified in this study in different demographic groups will require further studies now facilitated by knowledge of their genomes.

    PMID: 19211756 [PubMed - as supplied by publisher]



    More...

Latest Articles

Collapse

  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM
  • seqadmin
    The Impact of AI in Genomic Medicine
    by seqadmin



    Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
    02-26-2024, 02:07 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-14-2024, 06:13 AM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-08-2024, 08:03 AM
0 responses
71 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-07-2024, 08:13 AM
0 responses
80 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-06-2024, 09:51 AM
0 responses
68 views
0 likes
Last Post seqadmin  
Working...
X