Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • A question about install RSEQtools

    Hi everyone,

    I am interesting in RSEQtools,but I have some problem while install.

    In the begin,I have already installed gsl,and I download source code RSEQtools-0.6.tar.gz

    After I set environment variables,I cd to bios and keyin make,but show error as following:

    Makefile:6: /usr/local/bin//biosdefs.make: No such file or directory
    make: *** No rule to make target `/usr/local/bin//biosdefs.make'. Stop.


    My problem is I can't find biosdefs.make in my gsl,I think gsl 's version I installed is different as official.

    But I don't which version is official usage?

    Thanks!!!!!!

    Best Regard!

  • #2
    I am unable to use sam2mrf provided by prebuilt binaries of RSEQtools, can anybody pls help?
    The command I tried was "sort -r file.sam | sam2mrf > file.mrf"
    as specified in the manual for the alignment file generated by tophat but it didnt work.

    please reply soon.

    Comment


    • #3
      Hi,

      I had the same problem until I realized that I had to export the path variables from my bash.

      Previous .BASHRC settings:
      #RSEQtools
      BIOINFOCONFDIR=~/Programs/RSEQtools/bios/conf
      BIOINFOGSLDIR=~/Programs/gsl-2.4


      Changed to:
      #RSEQtools
      export BIOINFOCONFDIR=~/Programs/RSEQtools/bios/conf
      export BIOINFOGSLDIR=~/Programs/gsl-2.4

      [I]Settings were saved[\I]

      source ~/.bashrc
      make

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      27 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      30 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      26 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      52 views
      0 likes
      Last Post seqadmin  
      Working...
      X