I am aware of two Illumina kits that can do multiplexing: the "multiplexing sample preparation oligonucleotide kit" and the "TruSeq sample preparation kit". The steps between these procedures and the "ChIP-seq sample preparation kit" are qualitatively the same. However, it seems that the former two kits are designed for sequencing efforts that allow for large starting quantities of DNA (1-5ug). For ChIP-seq, you will be starting with more like 10ng.
The question is, has anyone done multiplexing for ChIP-seq? If so, which kit did you use? And, did you tweak the final concentrations of any of the steps to prevent a massive excess of adapters during the ligation?
Keith.
The question is, has anyone done multiplexing for ChIP-seq? If so, which kit did you use? And, did you tweak the final concentrations of any of the steps to prevent a massive excess of adapters during the ligation?
Keith.
Comment