So, I have a SureSelect custom designed assay and have calculated my % on target as around 45% using Picard CalculateHSMetrics. While this is helpful, it would be really interesting to know where the other 55% has aligned.
According to Picard, another 20% ish is near my target region, so what about the rest.
Is there a way of finding out to where the rest has aligned?
The sort of thing that I would be interested in is in any ?pseudogene areas or regions of homologous to my targeted regions with hot-spots of 'miss- aligned' reads...
Thanks
Chris
According to Picard, another 20% ish is near my target region, so what about the rest.
Is there a way of finding out to where the rest has aligned?
The sort of thing that I would be interested in is in any ?pseudogene areas or regions of homologous to my targeted regions with hot-spots of 'miss- aligned' reads...
Thanks
Chris
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