Dear All,
I am working on an Asian plant genus, and would like to use NGS to elucidate its phylogeography and evolutionary relations. For this I was thinking of using the NGS data as primary data (as shown in a paper on bird divergences by J. McCormack, 2012).
The question remains however which platform to use:
I know 454 produces longer reads, but is the more expensive option (100 specimens, means 100 MID tags, and 100 adaptors...)
Illumina might leave me with short reads, forcing me to analyze SNPs instead of sequences (some analyses I will use do not work with SNPs).
I would appreciate any help in making this decision.
Regards,
Yannick
I am working on an Asian plant genus, and would like to use NGS to elucidate its phylogeography and evolutionary relations. For this I was thinking of using the NGS data as primary data (as shown in a paper on bird divergences by J. McCormack, 2012).
The question remains however which platform to use:
I know 454 produces longer reads, but is the more expensive option (100 specimens, means 100 MID tags, and 100 adaptors...)
Illumina might leave me with short reads, forcing me to analyze SNPs instead of sequences (some analyses I will use do not work with SNPs).
I would appreciate any help in making this decision.
Regards,
Yannick