Hello
I was recently told that that FASTQ data from Illumina 1.8 or higher no longer has to be converted to Sanger FASTQ. Furthermore, that FASTQ Quality Filter is not necessary either because TopHat takes care of that and filters low quality reads.
I just wanted to be sure that this is correct.
Any input would be appreciated.
EBER.
I was recently told that that FASTQ data from Illumina 1.8 or higher no longer has to be converted to Sanger FASTQ. Furthermore, that FASTQ Quality Filter is not necessary either because TopHat takes care of that and filters low quality reads.
I just wanted to be sure that this is correct.
Any input would be appreciated.
EBER.