I'm wondering if anyone has data on the approximate yield for a single cluster during bridge amplification (i.e. ng/cluster for a theoretical 100bp target). In the context of liquid PCR I imagine it's limited due to physical contraints vs. diffusion but if anyone has data on this, I'd be really interested. thanks!
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This Illumina document (PDF) says "up to 1000 identical molecules" of each DNA per cluster, so sub-femtogram.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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