Dear all,
I'm having the error message while running Tophat with the following command:
My input data is human transcriptome, Illumina pair-end read with 14Gb of left and right read each, total input file size is 24Gb, insert size is 250.
My server got 128Gb RAM, 1Tb free space to run tophat and 64 cpu.
I just not sure how this error will occur
My command work perfectly nice for small set of data and test sequence.
Thanks and looking forward to hear from everybody.
I'm having the error message while running Tophat with the following command:
Code:
tophat -o tophat_run --bowtie1 -p 15 -G hg_19.gtf -r 250 --library_type fr-unstranded -M hg19 1.fastq 2.fastq > run_log 2> run_error_log
Code:
[2012-08-17 15:11:17] Reporting output tracks [FAILED] Error running tophat_reports --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir tophat_run/ --max-multihits 20 --max-seg-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --max-mismatches 2 --max-insertion-length 3 --max-deletion-length 3 --bowtie1 -z gzip -p15 --inner-dist-mean 250 --inner-dist-std-dev 20 --gtf-annotations hg_19.gff --gtf-juncs tophat_run/tmp/hg_19.juncs --no-closure-search --no-coverage-search --no-microexon-search --library-type fr-unstranded --sam-header tophat_run/tmp/hg19_genome.bwt.samheader.sam --samtools=samtools --bowtie2-max-penalty 6 --bowtie2-min-penalty 2 --bowtie2-penalty-for-N 1 --bowtie2-read-gap-open 5 --bowtie2-read-gap-cont 3 --bowtie2-ref-gap-open 5 --bowtie2-ref-gap-cont 3 hg19.fa tophat_run/junctions.bed tophat_run/insertions.bed tophat_run/deletions.bed tophat_run/fusions.out tophat_run/tmp/accepted_hits tophat_run/tmp/left_kept_reads.m2g_um.candidates_and_unspl.bam tophat_run/tmp/left_kept_reads.bam tophat_run/tmp/right_kept_reads.m2g_um.candidates_and_unspl.bam tophat_run/tmp/right_kept_reads.bam Loaded 288111 junctions
My server got 128Gb RAM, 1Tb free space to run tophat and 64 cpu.
I just not sure how this error will occur
My command work perfectly nice for small set of data and test sequence.
Thanks and looking forward to hear from everybody.