Does anyone happen to know the concentration of the Illumina PE adapters?
I would like to calculate the amount of adapter to add to ligations where I have less than the recommended amount of A-tailed library DNA, in order to maintain the ratio at 10:1 or 20:1. However the concentration of the adapter mix is not stated!
I have the same problem with the Agilent InPE (index paired end) adapter mix for SureSelect - I cannot find the concentration of the adapter mix.
Thanks,
Gavin
I would like to calculate the amount of adapter to add to ligations where I have less than the recommended amount of A-tailed library DNA, in order to maintain the ratio at 10:1 or 20:1. However the concentration of the adapter mix is not stated!
I have the same problem with the Agilent InPE (index paired end) adapter mix for SureSelect - I cannot find the concentration of the adapter mix.
Thanks,
Gavin
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