Dear All,
I have a fastq file which failed to align with bowtie. The error message is: reads file does not look like a FASTQ file. I don't know what is going wrong. Is it possible that there is no index in the fastq file? This is how my fastq file looks like:
@D5N3XBQ1:149:C0TCLACXX:2:1101:1342:2245 1:N:0:
TTAATAAAAGCTATTGAT
+
BB**?BHGG9?0?8888B
@D5N3XBQ1:149:C0TCLACXX:2:1101:1583:2228 1:N:0:
ATCGGGCGCAGGGACAGA
+
E99)?0?@6;'5>(;BE@
@D5N3XBQ1:149:C0TCLACXX:2:1101:1688:2238 1:N:0:
GCTGCCCTCCACCATATC
Thanks for all your ideas,
Lisanne
I have a fastq file which failed to align with bowtie. The error message is: reads file does not look like a FASTQ file. I don't know what is going wrong. Is it possible that there is no index in the fastq file? This is how my fastq file looks like:
@D5N3XBQ1:149:C0TCLACXX:2:1101:1342:2245 1:N:0:
TTAATAAAAGCTATTGAT
+
BB**?BHGG9?0?8888B
@D5N3XBQ1:149:C0TCLACXX:2:1101:1583:2228 1:N:0:
ATCGGGCGCAGGGACAGA
+
E99)?0?@6;'5>(;BE@
@D5N3XBQ1:149:C0TCLACXX:2:1101:1688:2238 1:N:0:
GCTGCCCTCCACCATATC
Thanks for all your ideas,
Lisanne
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