hello,
I have been trying to do analysis of RNA seq data, using the protocol given by tapnell paper in 2012 >> http://www.nature.com/nprot/journal/...2012.016.html#
I was trying to build bowtieIndex using command specified in paper
command was..
$ ln –s ./Drosophila_melanogaster/Ensembl/BDGP5.25/Sequence/BowtieIndex/genome.*. \
bu I am getting error>>
ln: target `./Drosophila_melanogaster/Ensembl/BDGP5.25/Sequence/BowtieIndex/genome.rev.2.ebwt' is not a directory
Please help me to sort out so that I could run this command
tophat -p 8 -G genes.gtf -o C1_R1_thout genome C1_R1_1.fq C1_R1_2.fq
I have been trying to do analysis of RNA seq data, using the protocol given by tapnell paper in 2012 >> http://www.nature.com/nprot/journal/...2012.016.html#
I was trying to build bowtieIndex using command specified in paper
command was..
$ ln –s ./Drosophila_melanogaster/Ensembl/BDGP5.25/Sequence/BowtieIndex/genome.*. \
bu I am getting error>>
ln: target `./Drosophila_melanogaster/Ensembl/BDGP5.25/Sequence/BowtieIndex/genome.rev.2.ebwt' is not a directory
Please help me to sort out so that I could run this command
tophat -p 8 -G genes.gtf -o C1_R1_thout genome C1_R1_1.fq C1_R1_2.fq
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