I recently got back my first data set from a PacBio run and was wondering what are 'typical' values for the different productivity levels? Here's what I received:
4.8% ZMW Loading for Productivity 0
38.6% ZMW Loading for Productivity 1
56.6% ZMW Loading for Productivity 2
The productivity 2 value seemed pretty high to me - these will mostly be unusable reads unless one of the polymerases drops off pretty early in the run, correct?
Could this indicate anything about my sample prep?
Thanks for any insight
4.8% ZMW Loading for Productivity 0
38.6% ZMW Loading for Productivity 1
56.6% ZMW Loading for Productivity 2
The productivity 2 value seemed pretty high to me - these will mostly be unusable reads unless one of the polymerases drops off pretty early in the run, correct?
Could this indicate anything about my sample prep?
Thanks for any insight
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