My core facility tells me that libraries should be above 3ng/ul for reliable quantification with the QuBit - mine are at 1ng/ul, which according to the manufacturer is perfectly within the assay range (HS dna assay).
I was wondering what was the general consensus on low concentration libraries - are they problematic / variable due to poor accuracy in the quantification? Should I quantify them by PCR or just reconcentrate them?
Thanks
I was wondering what was the general consensus on low concentration libraries - are they problematic / variable due to poor accuracy in the quantification? Should I quantify them by PCR or just reconcentrate them?
Thanks
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