Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • DNAjunk
    Member
    • Jun 2009
    • 62

    Problem with Combine Alignments

    Hi

    We're using Ion Proton with the latest Torrent Suite (3.4.1).
    When we would like to merge data from different runs belonging to the same project, then suddenly the alignment rate of the combined alignment drops to less than 20% although the alignment rates of the individual (not merged) runs were 95%.
    Has anyone seen this problem before and do you know the reason/solution ?
    Many thanks!
  • DNAjunk
    Member
    • Jun 2009
    • 62

    #2
    PS: It seems that the merged bam file is present and correct and only the presentation of the alignment (rate in percentage and curve) is wrong in the torrent suite browser. Although the combine alignments process is terminated, the browser indicates "Still calculating."

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      Sounds like you need to get in touch with Ion Proton support. Not many on this group have Ion Protons yet.

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 07-02-2026, 11:08 AM
      0 responses
      25 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      24 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      23 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      55 views
      0 reactions
      Last Post SEQadmin2  
      Working...