Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • GATK -rf BadCigar (Reads starting with deletions)

    Please excuse any naive or ignorant questions here, but I'm still very new to bioinformatics. I'm trying to run some illumina sequence data through the GATK pipeline and have been running into problems with reads beginning with deletions. I've been running some initial commands on my .bam files to make sure GATK is OK with the files and while I can run CountReads successfully, I get a BadCigar error return when I run the CountLoci command. Of the 3 data files I'm looking at, all three return BadCigar errors, and all 3 errors are related to reads starting with deletions.

    While I can run these files successfully by using the '-rf BadCigar' flag, this use raises a couple of questions:

    1.) Why does GATK consider these reads to be in error when we have yet to map the InDels and conduct a realignment?

    2.) The process of using the BadCigar flag seems to ignore the entire read, which is not ideal for my purposes. Is it possible to force the analysis to run and simply permit the bad cigars rather than ignore the entire read?

    3.)The GATK doesn't seem to report which reads have been ignored (beyond the initial run of the file not using the flag, in which it error's out at the first bad cigar). Is it possible to have the GATK report each bad cigar as it is ignored in the analysis?

    Thanks to anyone who can shed some light on these questions.

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
27 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
31 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
27 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
52 views
0 likes
Last Post seqadmin  
Working...
X