Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Covaris/Hydroshear

    Hi,

    I know the topic has been discussed before... but we are about to test Covaris and Hydroshear systems.
    I would be interested to hear some experiences of people shearing their DNA with one of those. We do mainly ChIP-Seq, having started in February this year.
    So far we have been using a Bioruptor but several researchers have had trouble getting their samples down to a reasonable size.

    Thanks in advance for any responses and by the way thanks to the ones who run this helpful and informative website.

    Cheers
    Andibody

  • #2
    I'm sorry that I can't speak about the hydroshear, but I have a colleague who has been testing genomic dna on Covaris vs Bioruptor. There really isn't much of a difference in fragment size so we're planning to go with Bioruptor since it's cheaper and you can process multiple samples.
    I've been testing the Bioruptor XL (the larger one that can do up to 48 samples at a time) on long range pcr products and so far it hasn't worked consistently. I've heard from others that it also doesn't work on genomic dna. However, we will be getting the Bioruptor 200 and have briefly tested that before and it works.
    There is another thread here about this. http://seqanswers.com/forums/showthr...ight=bioruptor

    Can you tell me which Bioruptor system you're using? And what the problems have been...what fragment sizes you're getting? I'd be interested to see what you've tried as I've been varying time and concentration.

    best...

    Comment


    • #3
      Hydroshear replacement from covaris



      Covaris consumable shears DNA to 6kbp - 20kbp fragments
      g-TUBE, the latest innovation from Covaris, is a single-use consumable that enables scientists to shear genomic DNA into selected fragments sizes ranging from 6kbp to 20kbp. The only equipment needed is a compatible bench-top centrifuge and a pipette.

      g-TUBE uses centrifugal force (the "g" in g-TUBE) to push the sample through a precisely manufactured orifice in the embedded ruby. This produces shearing forces in the sample that fragment the DNA. Fragment size is selected by adjusting the centrifuge rotor speed which alters the flow rate through the ruby and thus the shearing forces. Higher centrifugation RPM will produce shorter fragments and the protocol provide by Covaris contains the settings needed to select a fragment size between 6kbp and 20kbp.

      Remarkably, the entire process takes only 3 minutes and up to 24 samples can be processed simultaneously.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      51 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      45 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X