Hi,
My problem regards RNA-Seq data. I've downloaded public data (SAGE libs w/ 6 different samples from mouse liver ) to analyse using ArrayStudio. When I try to map them on the B38 mus musculus genom I have an unmapped read % of approximatly 95 % on all the samples!!! Quality scores are correct around 40 read length is correct (35 bp) but the base distrib QC is just very heterogenous, I don't understand why... this the first time I work on mouse data.Does anybody shared the same problem or have an idea please regarding the mapping and/or the base distrib?
Thanks, LN
My problem regards RNA-Seq data. I've downloaded public data (SAGE libs w/ 6 different samples from mouse liver ) to analyse using ArrayStudio. When I try to map them on the B38 mus musculus genom I have an unmapped read % of approximatly 95 % on all the samples!!! Quality scores are correct around 40 read length is correct (35 bp) but the base distrib QC is just very heterogenous, I don't understand why... this the first time I work on mouse data.Does anybody shared the same problem or have an idea please regarding the mapping and/or the base distrib?
Thanks, LN
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