Hi,
I made some control TruSeq genomic gel-free libraries using Ecoli and pooled these together equimolar. This pool then underwent blue pippin size selection at 540bp. The plot after duplicate marking in the post-sequencing analyses is attached to this email as well as the Agilent trace pre-sequencing/post-sizing. Bimodal size distribution was noted despite the Agilent trace looking quite clean before sequencing. Does anyone have any thoughts on how to improve this?
Thanks,
Anna.
I made some control TruSeq genomic gel-free libraries using Ecoli and pooled these together equimolar. This pool then underwent blue pippin size selection at 540bp. The plot after duplicate marking in the post-sequencing analyses is attached to this email as well as the Agilent trace pre-sequencing/post-sizing. Bimodal size distribution was noted despite the Agilent trace looking quite clean before sequencing. Does anyone have any thoughts on how to improve this?
Thanks,
Anna.