Hi all,
I have used the Illumina TruSeq Chip Kit for library preparation and just got the disappointing results.
I get an incredibly high enrichment of AT rich sequences, basically satellite repeats. Unfortunately this happens also in the input samples.
Could it be a bias in the amplification- but why?
Any ideas would be greatly appreciated!!
Thanks,
Sabine
I have used the Illumina TruSeq Chip Kit for library preparation and just got the disappointing results.
I get an incredibly high enrichment of AT rich sequences, basically satellite repeats. Unfortunately this happens also in the input samples.
Could it be a bias in the amplification- but why?
Any ideas would be greatly appreciated!!
Thanks,
Sabine
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