Hi, I am working on a set of miRNA TruSeq data. I did some search here and have decided to try TMM and DESeq for normaliztion. There are 3 biological repeats for each condition, but when the core facility ran the experiment, each sample was split into multiple lanes/flow cells. My question is what should I do with these technical repeats. My intention is to sum those up so that I have one value per gene for each biological sample, and normalize on that. But I don't know how it is different from normalization across all the technical/biological repeats.
Thank you for any input.
Thank you for any input.
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