Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Flow cell/channel terminology

    Hello all,

    I am a little bit perplexed of the exact mening of the term 'channel' as it is used by Helicos. So, I understand that there machine has two flow cells - in one the synthesis reaction takes place whilst the other is being imaged and this switches back and forth for each added nucleotide. However, each flow cell has 25 independent channels; what does that mean? Does each channel correspond to a picture like this one? Basically, if the HeliScope was a qPCR machine, would it correspond to it being able to run two multiwell plates at the time, with 25 wells per plate?

    Edit: Just found a footnote in their product specification sheet stating that for more than 50 samples multiplexing or barcoding is required. This would mean that I am right, right?
    Last edited by Calico; 01-25-2010, 08:14 PM. Reason: Gathered new information.

  • #2
    I think you are right that each channel is a independent picture for a scanner to photo. So it is similar with illumina GAs.
    Xi Wang

    Comment


    • #3
      Looking at the image on the Helicos website may help you -- though I'd suggest any future photographers of these load them up with colored dye to get some higher contrast. Each channel is more than a separate area to photograph, but is an actual separately microfabricated liquid holding region.

      Comment


      • #4
        Sorry I mixed something up. Channel for Helicos may equal to Lane for Illumina.
        Xi Wang

        Comment


        • #5
          Allright, that photo at least clarifies why they call them channels (i.e. they are channels). Though, now I am uncertain what the picture I posted is - a whole flow cell or a segment of a single channel?

          Comment


          • #6
            illumina calls that small square tile. a channel (lane) is made up by several (120 for illunima, 60 rows X 2 cols) tiles.
            Xi Wang

            Comment


            • #7
              And that would be the case for Helicos as well?
              Are they imaged sequentially or all simultaneously (in that case, all ties of one channel or of one flow cell at the same time)?

              Comment


              • #8
                sorry, my typo, it's tile, not tie.
                they are imaged sequentially. and it is the most time consuming step.
                Xi Wang

                Comment

                Latest Articles

                Collapse

                • seqadmin
                  Techniques and Challenges in Conservation Genomics
                  by seqadmin



                  The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                  Avian Conservation
                  Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                  03-08-2024, 10:41 AM
                • seqadmin
                  The Impact of AI in Genomic Medicine
                  by seqadmin



                  Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
                  02-26-2024, 02:07 PM

                ad_right_rmr

                Collapse

                News

                Collapse

                Topics Statistics Last Post
                Started by seqadmin, 03-14-2024, 06:13 AM
                0 responses
                33 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 03-08-2024, 08:03 AM
                0 responses
                72 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 03-07-2024, 08:13 AM
                0 responses
                81 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 03-06-2024, 09:51 AM
                0 responses
                68 views
                0 likes
                Last Post seqadmin  
                Working...
                X