Not a bioinformatician in the slightest, but need to start wearing that hat.
Had my last round of SELEX sequenced w/ Illumina Hi-Seq, and now I've got no idea how to go about processing it.
I know what the length and end sequences should be (primers) but don't have a sequence to align to, and that's what the majority of help I've been able to find talks about.
I guess I need to trim and filter the reads so I can pick the top __ most abundant (depending on how many individual sequences were found)? Just looking for some direction.
Any help most appreciated!
Had my last round of SELEX sequenced w/ Illumina Hi-Seq, and now I've got no idea how to go about processing it.
I know what the length and end sequences should be (primers) but don't have a sequence to align to, and that's what the majority of help I've been able to find talks about.
I guess I need to trim and filter the reads so I can pick the top __ most abundant (depending on how many individual sequences were found)? Just looking for some direction.
Any help most appreciated!
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