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  • Hands-On Training in Transcript Reconstruction

    Discovering standard and non-standard RNA transcripts - How to detect canonical splicing, circular RNAs, trans-splicing, and fusion transcripts

    When?
    October 23rd - 24th 2014

    Where?
    Leipzig, Germany


    Scope and Topics
    The purpose of this workshop is to get a deeper understanding in the usage of split-read mapping in order to find splice junctions, predict new isoforms and uncover non-standard RNA molecules, like circularized RNAs or fusion-transcripts. Advantages and disadvantages of the so-called split-reads and their implications on data analyses will be covered. The participants will be trained to understand the mapping approach, to find potential problems/errors and finally to implement their own pipelines. After this course they will be able to find and analyze (non-)standard exon-exon junctions and create ready-to-use analyses pipelines.

    By the end of this workshop the participants will:
    • understand the implications of splicing or fusion events and the concept of split-reads
    • understand how to detect splice sites using split-read information
    • know how to predict and quantify different isoforms of genes
    • be able to find circularized RNAs or fusion-stranscripts
    • be able to perform differential splicing analyis
    • automate tasks with shell scripting to create reusable data pipelines
    • plot and visualize results
    • be able to reuse all analyses


    Find more information at http://www.ecseq.com/workshops/workshop_2014-04.html
    ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).

  • #2
    Combine the transcriptome reconstruction workshop with one of our other courses and you will get a 10% discount to all booked workshops!
    ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).

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